Identification of a new family of enzymes: amine dehydrogenases (AmDHs)

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    • PI: Carine Vergne-Vaxelaire

    We are looking for Amine dehydrogenases (AmDHs) with varied substrates/properties/structures within biodiversity to provide the biocatalysis community with alternatives to the other listed NAD(P)-dependent enzymes that also catalyse reductive amination.1

    In collaboration with G. Grogan (York University, UK) and the UMR teams with expertise in bioinformatics (link LABGEM, LAGE) and enzyme production (link LGBM), we have identified an AmDH family of more than 17,000 members. Their applications in synthesis in native or modified form are promising in view of the validations carried out at laboratory scale.2-10

    This work is supported by the MODAMDH (ANR-19-CE07-0007) and ALADIN (ESR/EquipEx+ ANR-21-ESRE-0021) projects.

      amine dehydrogenases

       

      amine dehydrogenases

       

      1. Ducrot L., Bennett M., Grogan G., Vergne-Vaxelaire C. NAD(P)H‐Dependent Enzymes for Reductive Amination: Active Site Description and Carbonyl‐Containing Compound Spectrum ; Adv. Synth. Catal., 2020, 363(2), 328-351; doi: 10.1002/adsc.202000870
      2. Mayol O., David S., Darii E., Debard A., Mariage A., Pellouin V., Petit JL., Salanoubat M., de Berardinis V., Zaparucha A., Vergne-Vaxelaire C., Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis; Catal. Sc. Technol. 2016, 6, 7421-7428; doi: 10.1039/c6cy01625a
      3. Mayol O., Bastard K., Beloti L., Frese A., Turkenburg J., Petit JL., Mariage A., Debard A., Pellouin V., Perret A., de Berardinis V., Zaparucha A., Grogan G., Vergne-Vaxelaire C., A family of native amine dehydrogenases for the asymmetric reductive amination of ketones; Nat. Catal., 2019, 2, 324-333; doi: 10.1038/s41929-019-0249-z
      4. Vergne-Vaxelaire C., de Berardinis V., Zaparucha A., WO2019008110
      5. Caparco A., A.Pelletier E., Petit JL., Jouenne A., Bommarius B., de Berardinis V., Zaparucha A., Champion J., Bommarius A., Vergne-Vaxelaire C., Metagenomic Mining for Amine Dehydrogenase Discovery; Adv. Synth. Catal., 2020, 362(12), 2427-2436; doi: 10.1002/adsc.202000094
      6. Bennett M., Ducrot L., Vergne-Vaxelaire C., Grogan G. Structure and Mutation of the Native Amine Dehydrogenase MATOUAmDH2; ChemBioChem, 2022, 23(10), e202200136; doi: 10.1002/cbic.202200136
      7. Ducrot L., Bennett M., Caparco A., Champion J., Bommarius A., Zaparucha A., Grogan G., Vergne-Vaxelaire C., Biocatalytic Reductive Amination by Native Amine Dehydrogenases to Access Short Chiral Alkyl Amines and Amino Alcohols; Frontiers in Catal., 2021, 1; doi: 10.3389/fctls.2021.781284
      8. Jongkind E., Fossey-Jouenne A., Mayol O., Zaparucha A., Vergne-Vaxelaire C., Paul E., Synthesis of Chiral Amines via a Bi-Enzymatic Cascade Using an Ene-Reductase and Amine Dehydrogenase; ChemCatChem, 2022, 14, e202101576; doi: 10.1002/cctc.202101576
      9. Ducrot L., Bennett M., André-Leroux G., Elisée E., Marynberg S., Fossey-Jouenne A., Zaparucha A., Grogan G., Vergne-Vaxelaire C., Expanding the Substrate Scope of Native Amine Dehydrogenases through In Silico Structural Exploration and Targeted Protein Engineering; ChemCatChem, 2022, 14(22), e202200880; doi: 10.1002/cctc.202200880
      10. Fossey-Jouenne A., Ducrot L., Jongkind E., Elisée E., Zaparucha A., Grogan G., Paul C., Vergne-Vaxelaire C., Native amine dehydrogenases can catalyze the direct reduction of carbonyl compounds to alcohols in the absence of ammonia; Frontiers in Catal., 2023, 3; doi: 10.3389/fctls.2023.1105948.

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